Essential Biochemistry - Enzymes (Section 5 - No. 36)
Noncompetitive inhibitors primarily impact enzyme function by:
Binding to the active site and preventing substrate binding.
Altering the enzyme's Km value.
Binding elsewhere on the enzyme, changing Vmax.
Decreasing the concentration of the substrate.
Explication
Noncompetitive inhibitors bind away from the active site, causing a change in Vmax.